GDNF (Rat) ELISA from MyBioSource.com

Supplier Page

Supplier Page from
MyBioSource.com for
GDNF (Rat) ELISA

Get Pricing

Description

Principle of the assay: The MyBioSource rat GDNF ELISA kit is based on standard sandwich enzyme-linked immune-sorbent as-say technology. Rat GDNF specific-specific polyclonal antibodies are precoated onto 96-well plates. The rat specific detection polyclonal antibodies are biotinylated. The unknowns and biotinylated detection antibodies are added to the wells subsequently and then followed by washing with prepared wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with prepared wash buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the rat GDNF amount of unknown captured in plate